Current Services (Staff Assisted for Regular Users)

Data Collection

• Cryo-EM imaging - low-dose, at liquid nitrogen temperature
• High image quality, high throughput
• Automated cryo-ET data collection
• Automated single particle data collection
• Remote data collection options

Sample Preparation

• Prepare cryo-EM grids using FEI Vitrobot freezing machine

Cryo Grid Screening

• Screen cryo grid conditions on auto-loader scope will save huge mount of time
• Screening will provide low mag atlas, medium mag montages for a few good meshes
• It will also take a few shots in each mesh

Image Processing

• Preliminary data processing for aligning movie frames in fly during data collection
• 3D reconstruction for tomography data
• 3D reconstruction of single particle data for regular sample such as icosahedral viruses

Training

• Basic theory of electron microscopy
• Hands-on training of basic TEM operation using existing EM Core Facility
• Advanced training on TEM operation using SerialEM
• Hands-on training for operating Titan Krios and Talos-Arctica
• Negative stain sample preparation
• Cryo-EM sample preparation